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wi38 normal human fibroblasts  (ATCC)


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    ATCC wi38 normal human fibroblasts
    Wi38 Normal Human Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wi38+normal+human+fibroblasts/WI-38/10__15376_slash_biores__21__2__2906___2924-80-16-20
    Average 99 stars, based on 3127 article reviews
    wi38 normal human fibroblasts - by Bioz Stars, 2026-10
    99/100 stars

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    other:

    Article Title: Induction of the activating transcription factor-4 in the intratumoral CD8+ T cells sustains their viability and anti-tumor activities
    Article Snippet: WI38 normal human fibroblasts, mouse MC38 colon adenocarcinoma cells and human HCT116 colorectal cancer cells were purchased from ATCC.

    Article Title: Ecofriendly production of CuO/ZnO nanocomposites from guava leaf biomass and their antimicrobial and selective anticancer activities
    Article Snippet: The cytotoxicity studies were performed on two cell lines: SKOV3 human ovarian carcinoma (ATCC® HTB-77TM) and Wi38 normal human fibroblasts (ATCC® CCL75TM).



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    FIGURE 1 Transcriptional landscape of <t>WI38</t> fibroblasts during the first 24 h of oncogenic HRASG12V overexpression. (A) Diagram describing the experimental approach for the time-resolved multi-omic profiling used to define the early gene-regulatory networks preceding OIS commitment. (B) Euler diagrams showing overlaps of differentially expressed genes (DEGs) for each time point in the time series relative to proliferating cells (0 h). (C) PCA projection plots showing the individual transcriptional trajectories of DEGs identified within 24 h of RAS induction. Two biologically independent time series are shown. (D) Heatmap showing the number-coded modules (I-IV) of DEGs identified within 24 h of RAS overexpression. Two biologically independent time series experiments are shown. (E) Functional over-representation analysis map showing significant associations of the Molecular Signatures Database (MSigDB) Hallmark gene sets for each module described in (D). Circle fill is color-coded according to the FDR-corrected p-value from a hypergeometric distribution test. Circle size is proportional to the percentage of genes in each MSigDB gene set found within each gene module. N > 200 genes per module. (F) PCA projection plots showing the trajectories of DEGs identified through the entire time series (up to 144 h after RAS overexpression). (G) (Continued)
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    FIGURE 1 Transcriptional landscape of <t>WI38</t> fibroblasts during the first 24 h of oncogenic HRASG12V overexpression. (A) Diagram describing the experimental approach for the time-resolved multi-omic profiling used to define the early gene-regulatory networks preceding OIS commitment. (B) Euler diagrams showing overlaps of differentially expressed genes (DEGs) for each time point in the time series relative to proliferating cells (0 h). (C) PCA projection plots showing the individual transcriptional trajectories of DEGs identified within 24 h of RAS induction. Two biologically independent time series are shown. (D) Heatmap showing the number-coded modules (I-IV) of DEGs identified within 24 h of RAS overexpression. Two biologically independent time series experiments are shown. (E) Functional over-representation analysis map showing significant associations of the Molecular Signatures Database (MSigDB) Hallmark gene sets for each module described in (D). Circle fill is color-coded according to the FDR-corrected p-value from a hypergeometric distribution test. Circle size is proportional to the percentage of genes in each MSigDB gene set found within each gene module. N > 200 genes per module. (F) PCA projection plots showing the trajectories of DEGs identified through the entire time series (up to 144 h after RAS overexpression). (G) (Continued)
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    FIGURE 1 Transcriptional landscape of <t>WI38</t> fibroblasts during the first 24 h of oncogenic HRASG12V overexpression. (A) Diagram describing the experimental approach for the time-resolved multi-omic profiling used to define the early gene-regulatory networks preceding OIS commitment. (B) Euler diagrams showing overlaps of differentially expressed genes (DEGs) for each time point in the time series relative to proliferating cells (0 h). (C) PCA projection plots showing the individual transcriptional trajectories of DEGs identified within 24 h of RAS induction. Two biologically independent time series are shown. (D) Heatmap showing the number-coded modules (I-IV) of DEGs identified within 24 h of RAS overexpression. Two biologically independent time series experiments are shown. (E) Functional over-representation analysis map showing significant associations of the Molecular Signatures Database (MSigDB) Hallmark gene sets for each module described in (D). Circle fill is color-coded according to the FDR-corrected p-value from a hypergeometric distribution test. Circle size is proportional to the percentage of genes in each MSigDB gene set found within each gene module. N > 200 genes per module. (F) PCA projection plots showing the trajectories of DEGs identified through the entire time series (up to 144 h after RAS overexpression). (G) (Continued)
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    FIGURE 1 Transcriptional landscape of <t>WI38</t> fibroblasts during the first 24 h of oncogenic HRASG12V overexpression. (A) Diagram describing the experimental approach for the time-resolved multi-omic profiling used to define the early gene-regulatory networks preceding OIS commitment. (B) Euler diagrams showing overlaps of differentially expressed genes (DEGs) for each time point in the time series relative to proliferating cells (0 h). (C) PCA projection plots showing the individual transcriptional trajectories of DEGs identified within 24 h of RAS induction. Two biologically independent time series are shown. (D) Heatmap showing the number-coded modules (I-IV) of DEGs identified within 24 h of RAS overexpression. Two biologically independent time series experiments are shown. (E) Functional over-representation analysis map showing significant associations of the Molecular Signatures Database (MSigDB) Hallmark gene sets for each module described in (D). Circle fill is color-coded according to the FDR-corrected p-value from a hypergeometric distribution test. Circle size is proportional to the percentage of genes in each MSigDB gene set found within each gene module. N > 200 genes per module. (F) PCA projection plots showing the trajectories of DEGs identified through the entire time series (up to 144 h after RAS overexpression). (G) (Continued)
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    FIGURE 1 Transcriptional landscape of <t>WI38</t> fibroblasts during the first 24 h of oncogenic HRASG12V overexpression. (A) Diagram describing the experimental approach for the time-resolved multi-omic profiling used to define the early gene-regulatory networks preceding OIS commitment. (B) Euler diagrams showing overlaps of differentially expressed genes (DEGs) for each time point in the time series relative to proliferating cells (0 h). (C) PCA projection plots showing the individual transcriptional trajectories of DEGs identified within 24 h of RAS induction. Two biologically independent time series are shown. (D) Heatmap showing the number-coded modules (I-IV) of DEGs identified within 24 h of RAS overexpression. Two biologically independent time series experiments are shown. (E) Functional over-representation analysis map showing significant associations of the Molecular Signatures Database (MSigDB) Hallmark gene sets for each module described in (D). Circle fill is color-coded according to the FDR-corrected p-value from a hypergeometric distribution test. Circle size is proportional to the percentage of genes in each MSigDB gene set found within each gene module. N > 200 genes per module. (F) PCA projection plots showing the trajectories of DEGs identified through the entire time series (up to 144 h after RAS overexpression). (G) (Continued)
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    FIGURE 1 Transcriptional landscape of WI38 fibroblasts during the first 24 h of oncogenic HRASG12V overexpression. (A) Diagram describing the experimental approach for the time-resolved multi-omic profiling used to define the early gene-regulatory networks preceding OIS commitment. (B) Euler diagrams showing overlaps of differentially expressed genes (DEGs) for each time point in the time series relative to proliferating cells (0 h). (C) PCA projection plots showing the individual transcriptional trajectories of DEGs identified within 24 h of RAS induction. Two biologically independent time series are shown. (D) Heatmap showing the number-coded modules (I-IV) of DEGs identified within 24 h of RAS overexpression. Two biologically independent time series experiments are shown. (E) Functional over-representation analysis map showing significant associations of the Molecular Signatures Database (MSigDB) Hallmark gene sets for each module described in (D). Circle fill is color-coded according to the FDR-corrected p-value from a hypergeometric distribution test. Circle size is proportional to the percentage of genes in each MSigDB gene set found within each gene module. N > 200 genes per module. (F) PCA projection plots showing the trajectories of DEGs identified through the entire time series (up to 144 h after RAS overexpression). (G) (Continued)

    Journal: Frontiers in Epigenetics and Epigenomics

    Article Title: Transcription factor network dynamics during the commitment to oncogene-induced senescence

    doi: 10.3389/freae.2024.1423454

    Figure Lengend Snippet: FIGURE 1 Transcriptional landscape of WI38 fibroblasts during the first 24 h of oncogenic HRASG12V overexpression. (A) Diagram describing the experimental approach for the time-resolved multi-omic profiling used to define the early gene-regulatory networks preceding OIS commitment. (B) Euler diagrams showing overlaps of differentially expressed genes (DEGs) for each time point in the time series relative to proliferating cells (0 h). (C) PCA projection plots showing the individual transcriptional trajectories of DEGs identified within 24 h of RAS induction. Two biologically independent time series are shown. (D) Heatmap showing the number-coded modules (I-IV) of DEGs identified within 24 h of RAS overexpression. Two biologically independent time series experiments are shown. (E) Functional over-representation analysis map showing significant associations of the Molecular Signatures Database (MSigDB) Hallmark gene sets for each module described in (D). Circle fill is color-coded according to the FDR-corrected p-value from a hypergeometric distribution test. Circle size is proportional to the percentage of genes in each MSigDB gene set found within each gene module. N > 200 genes per module. (F) PCA projection plots showing the trajectories of DEGs identified through the entire time series (up to 144 h after RAS overexpression). (G) (Continued)

    Article Snippet: WI38 normal human lung fibroblasts (CCL-75, ATCC -American Type Culture Collection) and GM21808 normal human skin fibroblasts (Coriell Institute) were cultured in a DMEM (D6429, Millipore-Sigma) medium supplemented with 10% fetal bovine serum, (#25–514, GenClone) at 37°C in a 5% CO2 and 2% O2 atmosphere.

    Techniques: Over Expression, Functional Assay